Institutions dedicated to zoology are crucial in advancing the One Health movement's objectives. We predicted that Zoo members would be concerned about infectious disease issues and knowledgeable about One Health. The results of the survey revealed that Zoo members were particularly concerned about chronic, non-infectious diseases and the related financial expenses. Participants ranked nutrition/obesity/diet, health care costs, and cancer as their top three health issues of concern for humans. Zoo members expressed interest in and importance for zoos' engagement in One Health. According to these findings, zoos might do better to cater One Health messaging to members' interests. The Saint Louis Zoo's messaging and programming will now emphasise the direct health advantages that zoos provide in addition to the Institute for Conservation Medicine's (ICM) more ecologically oriented initiatives. This study provided insightful information about how Zoo members see One Health and might be used as a model to assist.