Biological processes using microorganisms have a wide range of advantages over conventional chemical processes for biofuel production (such as renewal, sustainability and carbon neutralization). However, apart from compatibility with existing fuel infrastructure, the production efficiency of modern biofuels is still insufficient to compete with and replace fossil fuels. The main challenges to be addressed in biological systems are substrate cost and inhibition of biofuel toxicity/ fermenting microorganisms, which are directly related to biofuel efficiency, title and yield. To overcome these obstacles, it is necessary to create a strong and productive microbiota. Recently various engineering microorganisms with the help of genetic engineering and artificial biological engineering with high-level productivity of biofilm production (e.g. ethanol, biodiesel, pitanol, at the base, synchronization and H2).
