Mahesh B. Narkhede, Nilesh M. Mahajan, Prashant V. Ajmire, Mangesh S. Jonwal
The object of study was to evaluate the use of isolated chicken intestine as an alternative preparation for dose response curve (DRC) and estimation of unknown concentration of acetylcholine (ACh). The DRC was performed and graded doses of ACh have shown dose dependent increase in response of contraction. The obtained responses of ACh on chicken intestine were similar to those where performed in isolated preparations. Also different experiment viz. cumulative DRC, potentiation effect, nature of antagonist, calculation of PA2 value were performed in our pharmacology laboratory. The results suggest that a chicken intestine preparation is suitable for the purpose experiment on isolated preparation and multipoint bioassay. The chicken intestine can be used successfully in routing pharmacology experiment in place of isolated preparation from frog, rodents and other experimental animals to perform or demonstrate the student for action of acetylcholine.
