Shirish P. Lokhande, Surya Prakash Gupta, K.Sureshkumar, J. Dharuman, Gopal Garg, Neeraj Upmanyu
A simple, precise, accurate and rapid high-performance thin-layer chromatographic method has been developed and validated for the estimation of ramipril and valsartan simultaneously in combined dosage form. The stationary phase used was precoated silica gel 60F254. The mobile phase used was a mixture of chloroform: ethyl acetate: methanol: glacial acetic acid (5.0:5.0:1.0:02 v/v/v/v). The detection of spots was carried out at 210 nm. The method was validated in terms of linearity, accuracy, and precision. The calibration curve was found to be linear between 0.4 to 2.0 μg/spot for ramipril and 0.2 to 1.0 μg/spot for valsartan. The limit of detection and the limit of quantification for ramipril were found to be 200 ng/spot and 640 ng/spot for ramipril and 100 ng/spot and 330 ng/spot respectively. The proposed method can successfully be used to determine the drug content of marketed formulations.
