Sabikunnahar J, Jalal U, Zubair, K. labu
Roots of Clerodendrum viscosum are profoundly used throughout the world due to its ethno-medicinal properties and folkloric use since the very beginning of medicinal treatment. Due to the potential disease preventive properties, we investigate the methanolic extracts of the roots of the plant for biological evaluation in vitro.The methanolic extract of Cl. viscosum roots was partitioned into ethyl acetate soluble fraction (ESF), petroleum ether soluble fraction (PSF), carbon tetrachloride soluble fraction (CTSF), chloroform soluble fraction (CSF) and aqueous soluble fraction (AQSF). The extracts were evaluated for their thrombolytic, membrane stabilizing, antimicrobial and the results were compared with standard drugs; streptokinase for thrombolytic, acetyl salicylic acid for anti-inflammatory, kanamycin for antimicrobial activities. In thrombolytic investigation, among all partitionates, the ESF showed highest % of clot lysis (54.47%) as compared to (69.13%) and (3.17%) exhibited by the standard streptokinase and water. In case of membrane stabilizing study, ESF also significantly inhibited the haemolysis of human erythrocyte membrane both induced by hypotonic solution (60.30 ± 0.54%) and by heat (44.21 ± 0.59%), respectively as compared to (69.01 ± 16%) and (71.90 ± 0.19%) demonstrated by acetyl salicylic acid. In antibacterial activity, it was exhibited by the extracts, was comparatively more prominent on the gram negative bacteria than the gram positive bacteria. Our study revealed that satisfactory amount of flavonoid and tannin presence showed a significant and positive correlation between bioactive compound contents with pharmacological activities of Cl. Viscosum roots.
