Aprilita Rinayanti , Maksum Radji, Abdul MunâÂÂim and F.D. Suyatna
In recent years the use of medicinal plants has been growing worldwide and this is particularly true in Indonesia. Ten parts from nine plants used by traditional healers in Indonesia for the treatment of high blood pressure were investigated for their antihypertensive properties, utilizing the angiotensin converting enzyme assay. Plant materials were macerated successively using petroleum ether, ethyl acetate and methanol. Organic solvent concentrated using rotary vacum evaporator yield petroleum ether extract (PEE), ethyl acetate extract (EAE) and methanolic extract (ME). ACE inhibitory activity was determined by spechtrophotometer and absorbance was read at 228 nm. The results show that leaves and fruits Phaleria macrocarpa (Scheff.) Boerl exhibited a strong inhibitory activity against ACE with IC50 in the leaves was 189.13 μg/ml in PEE, 157.74 μg/ml in EEA and 101.52 μg/ml ME, while the IC50 in the fruits was 161.7 μg/ml in PEE, 139.11 μg/ml in the EEA and 122.38 μg/ml in ME.
