Afif Ben Salah
Tunisia
Research Article
No Evidence of Interspecific Genetic Exchange by Multi-Locus Microsatellite Typing Between Leishmania Killicki and Leishmania Major in a Mixed Focus of Cutaneous Leishmaniasis in Southeast Tunisia
Author(s): Myriam Harrabi, Wissem Ghawar, Mallorie Hide, Jihène Bettaieb, Rihab Yazidi, Kaouthar Jaouadi, Chaâbane Sana, Bilel Chalgha, Amine Toumi, Amor Zaâtour, Mohamed Raouène, Anne-Laure Bañuls and Afif Ben SalahMyriam Harrabi, Wissem Ghawar, Mallorie Hide, Jihène Bettaieb, Rihab Yazidi, Kaouthar Jaouadi, Chaâbane Sana, Bilel Chalgha, Amine Toumi, Amor Zaâtour, Mohamed Raouène, Anne-Laure Bañuls and Afif Ben Salah
Sixty-four Leishmania samples were isolated from patients in several villages in the Tataouine governorate, southeast Tunisia. This region is known to be a mixed focus of human cutaneous Leishmaniasis caused by Leishmania (L.) killicki (synonymous L. tropica) and L. major. To identify the Leishmania species in this governorate, a nested polymerase chain reaction based on the variable region of the kinetoplast minicircle was performed on each isolate. Multi-locus microsatellite typing using markers selected for their ability to amplify the two species was used to explore patterns of interspecific genetic exchange. Thirteen L. major and 51 L. killicki isolates were identified. The analysis of microsatellite data showed very low genetic diversity in each species with this set of microsatellites but a high differentiation between the two species. Nine L. major and five L. killicki strains.. View More»
