Ahmad Baraka
Egypt
Research Article
Detection of Minimal Residual Disease in Childhood B-Acute Lymphoblastic Leukemia by 4-Color Flow Cytometry
Author(s): Ahmad Baraka, Laila M Sherief, Nagla M Kamal and Shereen El ShorbagyAhmad Baraka, Laila M Sherief, Nagla M Kamal and Shereen El Shorbagy
Monitoring of minimal residual disease (MRD) is today considered the most powerful predictor of outcome in acute leukemias, including acute lymphoblastic leukemia (ALL). The study aimed to determine whether panel of antibodies combination are more suitable than others for detection of MRD in Childhood B-lineage ALL. Eighty four (84) patients of ALL (B-lineage subtype) were enrolled in this study. Normal template for B. Cell precursors were been established in 15 control Patients by using 4 panels of monoclonal Abs (Mo Abs), {CD22, CD45, CD58 and CD97 in combination with CD10, CD19, CD34}. At diagnosis CD22 having the lowest incidence expression between the patients in 50% only, but CD45, CD58, and CD97 were expressed in 80.9%, 52.3% and 92.8% respectively. Analysis of MRD was performed for each Mo Abs combination at day 0 and day 14 post induction of chemotherapy by 4-color flow cytomete.. View More»
