Amit R Agarwal
Tanzania
Research Article
Tobacco Smoke-Induced Alterations in Hepatic Lipid Profiles Demonstrated by Imaging Mass Spectrometry
Author(s): Suzanne M de la Monte, Ming Tong, Kavin Nunez, Emine Yalcin, Jared Kay, Amit R Agarwal and Enrique CadenasSuzanne M de la Monte, Ming Tong, Kavin Nunez, Emine Yalcin, Jared Kay, Amit R Agarwal and Enrique Cadenas
To account for differences in susceptibility to alcoholic liver disease (ALD), tobacco smoking should be evaluated as a potential cofactor given the very high percentage of heavy drinkers also smoke, and the NNK tobacco-specific nitrosamine was shown to cause steatohepatitis and exacerbate molecular and biochemical effects of alcohol on the liver. Since one of the key factors linked to ALD progression is dysregulated lipid metabolism, we examined effects of cigarette smoke (CS) exposures on hepatic lipid profiles using matrix-assisted laser desorption and ionization imaging mass spectrometry (MALDI-IMS). Adult male A/J mice were exposed to air (8 weeks; A8), CS for 4 (CS4) or 8 (CS8) weeks; or CS8 with 2 weeks recovery (CS8+R). MALDI-IMS demonstrated broad CS-associated reductions in hepatic phospholipids that were partly ameliorated by short-term recovery. Principal component analysis r.. View More»
