Ashling Holland
Ireland
Review Article
Comparative Proteomics for Studying Muscular Dystrophy: Intrinsic
Biological and Analytical Issues Associated with the Systematic Utilization of Tissue Specimens
Author(s): Ashling Holland and Kay OhlendieckAshling Holland and Kay Ohlendieck
Over the past decade, mass spectrometry-based proteomics has been instrumental for the detailed elucidation of pathobiochemical mechanisms involved in major neuromuscular diseases. Although the identification of musclederived proteins in biofluids is the main focus of diagnostic biomarker research, the large-scale proteomic analysis of pathological muscle tissue is of central importance for furthering our general understanding of the dysregulation that underlies complex muscle diseases. Here, we discuss intrinsic biological issues and bioanalytical difficulties that are generally associated with comparative muscle tissue proteomics. The systematic utilization of cellular mixtures or whole tissue specimens as starting material for studying neuromuscular pathology is seriously complicated by the cellular heterogeneity and physiological plasticity of contractile tissues. The comprehensiv.. View More»
DOI: 10.4172/jpb.S10-002
