Biswaranjan Paital
Orissa University of Agriculture and Technology, College of Basic Science and Humanities,
Bhubaneswar, 751003, Odisha
India
Review Article
Mass Spectrophotometry: An Advanced Technique in Biomedical Sciences
Author(s): Biswaranjan PaitalBiswaranjan Paital
Present century is the era of life sciences and metabolomics is the dominating field in present day over genomics and proteomics. Powerful techniques in the later cases are useful to determine the qualitative and quantitative studies of the levels of metabolites in bio-medical samples. In past time, biochemistry had very least role because the analyses of metabolites using individual conventional biochemical techniques had been used from past centuries. Owing to this limitation, mass spectrometry (MS) was introduced in bio-medical sciences. The fundamental rule in MS is that it ionizes individual chemical species and sorts the fragmented samples at charged state (ions) on the basis of their mass to charge (m/z) ratio. Therefore, each molecule in purified form or in homogenate can be identified and quantified based on their unique m/z ratio. In other words, MS measures the masses of th.. View More»
