Dowell K
United Kingdom
Editorial
Assessment of 19,803 Paired Chromosomes and Clinical Outcome from First 150 Cycles using Array CGH of the First Polar Body for Embryo Selection and Transfer
Author(s): Fishel S, Craig A, Lynch C, Dowell K, Ndukwe G, Jenner L, Cater E, Brown A, Gordon A, Thornton S, Campbell A, Berrisford K, Kellam L and Sedler MFishel S, Craig A, Lynch C, Dowell K, Ndukwe G, Jenner L, Cater E, Brown A, Gordon A, Thornton S, Campbell A, Berrisford K, Kellam L and Sedler M
Background: Meiosis 1 errors are believed to be the largest single cause of clinical embryo failure and early miscarriage. Following the failure of FISH technology and concerns over embryo mosaicism, our aim was to assess paired chromosome status and error rates, and predict oocyte aneuploidy using the metaphase2 polar body with a novel Array CGH platform as a means to select embryos for couples with multiple IVF failures.
Methods: The PB1 was removed from metaphase 2 oocytes using a laser-assisted breech of the zona pellucida to effect the biopsy. Reliable 23-paired chromosome analysis was obtained, and in time for fresh embryo transfer. It was a non- randomised investigation in patients seeking this technology as a result of previous multiples failures of IVF.
Results: 134 couples presented for 150 cycles of arra.. View More»
