Research Article
A Simple and Efficient Methodology for the Study of Cardioprotective Drugs in Animal Model of Cardiac Ischemia-Reperfusion
Author(s): José Gustavo Padrão Tavares, Francisco Sandro Menezes-Rodrigues, Ênio Rodrigues Vasques, Maria do Carmo Maia Reis, Luciana de Paula, Bráulio Luna-Filho, Paolo Ruggero Errante, Afonso Caricati-Neto and Leandro Bueno BergantinJosé Gustavo Padrão Tavares, Francisco Sandro Menezes-Rodrigues, Ênio Rodrigues Vasques, Maria do Carmo Maia Reis, Luciana de Paula, Bráulio Luna-Filho, Paolo Ruggero Errante, Afonso Caricati-Neto and Leandro Bueno Bergantin
To study cardioprotective drugs, we developed a simple and efficient methodology to evaluate effects of drugs on cardiac electrical activity using electrocardiogram (ECG) in rats submitted to cardiac ischemia-reperfusion (I/R). Using adult male Wistar rats (14 - 16-week-old) anesthetized (urethane 1.25 g/kg, i.p.) and kept under mechanical ventilation, we used surgical procedures to induce cardiac I/R by means mechanical occlusion of left anterior descendent coronary artery for 10 min (ischemia) with silk suture (tourniquet) followed by its removal to allow coronary recirculation (reperfusion). To evaluate the effects of surgical process, a group of rats (SHAM-operated) was submitted to surgical procedures previously described, but without coronary occlusion. To evaluate cardiac electrical activity in rats submitted to cardiac I/R and SHAM-operated, the ECG system was coupled to anima.. View More»
