Journal of Multiple Sclerosis
ISSN - 2376-0389NLM - 101654564
Ghasem Ahangari
National Institute of Genetic Engineering and Biotechnology,
Tehran
West Indies
Research Article
New Discernment of Pathophysiological Aspects of Multiple Sclerosis Based On Mono Amino Oxidase (MAO) and Ion Channel Receptor 5HT3RA as Activator of T-Cells
Author(s): Toktam Deylami, Mohammad Hossein Sanati and Ghasem AhangariToktam Deylami, Mohammad Hossein Sanati and Ghasem Ahangari
Background: Multiple sclerosis (MS) is a chronic, inflammatory and autoimmune disease of central nervous system. MS affects nearly 2.5 million people in the world and is twice more common in women than men. Autoimmune T-cells target the myelin sheath in central nervous system, causing inflammation, demyelination and eventual destruction of neurons. We examined changing expression of serotonin receptor (5-HT3RA) as well as monoamine oxidase (MAO-A) genes in peripheral blood mononuclear cells in MS patients.
Materials and methods: In this study, peripheral blood mononuclear cells (PBMC) were first isolated from 30 healthy controls and 30 volunteers with MS using Ficoll-hypaque. Total RNA was extracted and cDNA was synthesized. In this process, mRNA concentration of 5-HT3RA and MAO-A as target genes as .. View More»
