Hae Young Ko
Republic of Korea
Research Article
Molecular Beacon-Based Microrna Biosensor for Imaging EPC-Treated Cellular Therapy of Ischemia
Author(s): Chang Hyun Lee, Jung il Chae, Hae Young Ko and Soonhag KimChang Hyun Lee, Jung il Chae, Hae Young Ko and Soonhag Kim
Angiogenesis, the process of new blood vessel formation, is an important therapeutic target in cardiovascular and malignant diseases for diverse reasons. Molecular imaging for angiogenesis has been attracted due to the use of anti-angiogenic therapeutic drugs to treat tumours, and of therapeutic angiogenesis induction to treat vascular diseases. We developed a novel biosensor of imaging microRNA126 (mir126) expressed during angiogenesis using a miRNA Molecular Beacon (MB) composed of a stem loop-structured DNA complementary to mir126 and Cy5.5 (near infrared, NIR)-black hole quencher 2 (BHQ2) (mir126 NIR MB). Mir126 in cord blood-derived endothelial precursor cells (CB-EPCs) was highly expressed and more expressed after the wound healing. The quantitative and qualitative fluorescence intensity of the mir126 NIR MB was high in CB-EPC and significantly increased after the wound healing,.. View More»
