Hajime Kobayashi
Japan
Research Article
The Nicotinamide Adenine Dinucleotide (NAD)-Dependent Deacetylase Sirtuin-1 Regulates Chondrocyte Energy Metabolism through the Modulation of Adenosine Monophosphate-Activated Protein Kinase (AMPK) in Osteoarthritis(OA)
Author(s): Hajime Kobayashi, Koh Terauchi, Naoko Yui, Kanaka Yatabe, Toshikazu Kamada, Hiroto Fujiya, Hisateru Niki, Haruki Musha and Kazuo YudohHajime Kobayashi, Koh Terauchi, Naoko Yui, Kanaka Yatabe, Toshikazu Kamada, Hiroto Fujiya, Hisateru Niki, Haruki Musha and Kazuo Yudoh
To clarify how the osteoarthritis (OA)-induced catabolic factor interleukin (IL)-1β affects chondrocyte energy metabolism, and especially to define the downstream pathway linking nicotinamide adenine dinucleotide (NAD)- dependent deacetylase Sirtuin-1 (Sirt-1) to energy metabolism in OA chondrocytes. Human chondrocytes were isolated from articular cartilage samples of patients with OA. The level of energy metabolism of OA chondrocytes was evaluated by monitoring the activity of the energy metabolic sensor, adenosine monophosphate-activated protein kinase (AMPK) and the level of production of adenosine triphosphate (ATP) in chondrocytes in the presence or absence of t IL-1β (10 ng/mL). Effects of IL-1β on anabolic and catabolic activities of chondrocytes were analyzed by the levels of production of proteoglycan and matrix metalloproteinase (MMP)-13, respectively. Experim.. View More»
