Hwang KS
Republic of Korea
Research Article
Cflarb Complemented the Function of Cflara to Allow Cflara Knock out Zebrafish To Normal Development
Author(s): Huh SJ, Hwang KS, Koppula S, Kim CG, Kim CH and Kim CGHuh SJ, Hwang KS, Koppula S, Kim CG, Kim CH and Kim CG
Cellular FLICE-inhibitory protein (cFLIP, cflara) is a regulator of death receptor (DR)-induced apoptosis and NF-κB activation. cFLIP is known to prevent activation of the caspase cascade by binding to FADD/caspase-8. Up-regulated cFLIP has been identified in many tumor types, and therefore restoring apoptosis by silencing cFLIP may be one of the more potent strategies in cancer therapeutics. The zebrafish cFLIP gene, cflara, has 2 death effector domains (DEDs) and a single caspase-like domain. Expression of cflara was detected in the zebrafish embryo by RT-PCR and whole-mount in situ hybridization. To study the in vivo function of cflara, we generated a cflara knockout mutant zebrafish using transcription activator-like effector nucleases (TALENs). Frame shift mutation is caused by a 10-bp deletion in the first DED domain. By inbreeding the F1 generation, a homozygous mutant fis.. View More»
