Iku Utsunomiya
Japan
Research Article
Effects of IgM Anti-Galnac-GD1a Monoclonal Antibody on Neuromuscular
Transmission and Calcium Channel Binding in the Rat Neuromuscular Junction
Author(s): Sayako Hotta, Takumi Nagaoka, Yoshihiko Nakatani, Toshie Kambe, Kenji Abe, Yutaka Masuda, Iku Utsunomiya and Kyoji TaguchiSayako Hotta, Takumi Nagaoka, Yoshihiko Nakatani, Toshie Kambe, Kenji Abe, Yutaka Masuda, Iku Utsunomiya and Kyoji Taguchi
Guillain-Barré syndrome with antibodies against ganglioside N-acetylgalactosaminyl GD1a (GalNAc-GD1a) is
characterized by a rapid onset of predominantly distal pure motor neuropathy. However, the pathogenic role of anti-
GalNAc-GD1a antibody and calcium channels in neuromuscular junctions (NMJs) remains unclear. We investigated
the effects of IgM anti-GalNAc-GD1a monoclonal antibody (IgM anti-GalNAc-GD1a mAb) on spontaneous muscle
action potentials (SMAP) in a rat spinal cord–muscle co-culture system and the localization of IgM anti-GalNAc-
GD1a mAb and calcium channel binding in the rat hemi-diaphragm. Immunohistochemistry of the rat hemidiaphragm
showed that IgM anti-GalNAc-GD1a mAb binding overlapped with anti-neurofilament 200 antibody and α-
bungarotoxin staining, demonstrating that IgM anti-GalNAc-GD1a mAb was l.. View More»
