Ilan Calderon
Israel
Research Article
Sphingosine-1-Phosphate may Decrease the Cytotoxic Effect of Doxorubicin on Human Granulosa Cells
Author(s): Orli Turgeman, Ilan Calderon, Martha Dirnfeld, Mada Hashem and Zeev BlumenfeldOrli Turgeman, Ilan Calderon, Martha Dirnfeld, Mada Hashem and Zeev Blumenfeld
Context: The increase in malignancy of young women in the recent decades, combined with a significant improvement in long term survival after gonadotoxic chemotherapy, have brought about a ubiquitous interest in preservation of fertility in these young patients. The present study examines the effects of Sphingosine-1-Phosphate (S1P) on primary human granulosa cell cultures in-vitro as a possible protecting factor against Doxorubicin (DOX) and Cyclophosphamide associated toxicity. Understanding cytotoxic effects and gonadotoxicity in human luteinized Granulosa Cells (GC) may contribute to our understanding and preventing follicle loss.
Study objective: To examine the possible protective effect of S1P on chemotherapy induced gonadotoxicity, in human luteinized Granulosa Cells (GCs).
Design: Human GC’s were donated by women undergoing follicular aspiration for in vi.. View More»
