Jan-Hendrik Streich
Germany
Research Article
Comparative Serum Proteomic Analysis of Differentially Regulated Proteins in Patients with Rheumatoid Arthritis and Healthy Volunteers
Author(s): Radovan Vasko, Sabine Blaschke, Jan-Hendrik Streich, Gerhard A Müller, Peter Korsten and Hassan DihaziRadovan Vasko, Sabine Blaschke, Jan-Hendrik Streich, Gerhard A Müller, Peter Korsten and Hassan Dihazi
Background: To identify differentially regulated serum proteins, we compared proteome profiles of sera from patients with rheumatoid arthritis (RA) and healthy controls using proteomics. Methods: Sera were collected from 43 patients with RA and 48 healthy volunteers. The samples were cleared of the most abundant major proteins by immunoaffinity chromatography. Serum protein profiles between the two groups were compared by two-dimensional differential gel electrophoresis (2D-DIGE) and differentially regulated proteins were studied using mass spectrometry. Results: We identified 26 differentially expressed serum proteins between patients with RA and healthy controls. A quantitatively significant change of protein levels was defined as at least 1.5-fold upregulation or 0.6-fold downregulation respectively. Using these criteria, patients with RA exhibited significantly higher levels of le.. View More»
