Jie Cheng
China
Research Article
Study on the Relationship between the Structure and Functions of Antihuman Cervical Cancer Single-chain Antibody and the Lengths of Linkers
Author(s): Jie Cheng, Quxiao Du, Xiaoli Zhang, Yafeng Ren, Boai Zhang and Xiaodong FengJie Cheng, Quxiao Du, Xiaoli Zhang, Yafeng Ren, Boai Zhang and Xiaodong Feng
Background: This study aimed to find the linker length with minimal impact among chains to fight against the structure and function of cervical cancer single-chain antibody.
Methods: The original variable region of heavy chain (VH) and variable region of light chain (VL), and the singlechain antibody with (Gly4Ser)n linkers of different lengths (n=1~8) were modeled using SWISS-MODEL homologous modeling. Comparison of the similarity of original VH/VL and VHn/VLn was carried out by applying the algorithm of spatial hierarchical alignment based on the spherical coordinates. The fore and aft distance and diffusion radius of α were also calculated. The stability of antibody with different linker length was then compared. ELISA method was adopted to evaluate the immunological activity of single-chain antibody with different linkers. MTT as.. View More»
DOI: 10.4172/jpb.S8-004
