Joanna S Kruszewska
Poland
Research Article
Toward the Quantification of Confrontation (Dual Culture) Test: A Case Study on the Biological control of Pythium aphanidermatum with Trichoderma asperelloides
Author(s): Babak S Pakdaman, Ebrahim Mohammadi Goltapeh, Bahram Mohammad Soltani, Ali Asghar Talebi, Mohsen Nadepoor, Joanna S Kruszewska, Sebastian Pilsyk, Sabrina Sarrocco and Giovanni VannacciBabak S Pakdaman, Ebrahim Mohammadi Goltapeh, Bahram Mohammad Soltani, Ali Asghar Talebi, Mohsen Nadepoor, Joanna S Kruszewska, Sebastian Pilsyk, Sabrina Sarrocco and Giovanni Vannacci
Trichoderma species are well-appreciated filamentous fungi applied in agriculture for biological control and biofertilization purposes. One of the primary steps to screen for potent biological control isolates and/to study the effect of gene(s) on the biological control of phytopathogenic fungi and oomycetes is to perform confrontation or dual culture tests. Despite the comprehensiveness of the test, it still suffers from the lack of a reliable methodology for the mathematical data collection and statistical analyses. Here, different aspects of data collection are critically studied and new parameters are introduced. With this method the statistical comparison of different fungal biological control isolates and their counterpart phytopathogenic fungi as well as oomycetes becomes feasible. In the mean time, with this new approach, it becomes possible to statistically analyze the effect.. View More»
