Julian P. Whitelegge
Tanzania
Research Article
Hydrophobic Fractionation Enhances Novel Protein Detection by Mass Spectrometry in Triple Negative Breast Cancer
Author(s): Ming Lu, Julian P. Whitelegge, Stephen A.Whelan, Jianbo He, Romaine E. Saxton, Kym F. Faull and Helena R. ChangMing Lu, Julian P. Whitelegge, Stephen A.Whelan, Jianbo He, Romaine E. Saxton, Kym F. Faull and Helena R. Chang
It is widely believed that discovery of specific, sensitive and reliable tumor biomarkers can improve the treatment of cancer. The goal of this study was to develop a novel fractionation protocol targeting hydrophobic proteins as possible cancer cell membrane biomarkers. Hydrophobic proteins of breast cancer tissues and cell lines were enriched by polymeric reverse phase columns. The retained proteins were eluted and digested for peptide identification by nano-liquid chromatography with tandem mass spectrometry using a hybrid linear ion-trap Orbitrap. Hundreds of proteins were identified from each of these three specimens: tumors, normal breast tissue, and breast cancer cell lines. Many of the identified proteins defined key cellular functions. Protein profiles of cancer and normal tissues from the same patient were systematically examined and compared. Stem cell mark.. View More»
DOI: 10.4172/jpb.1000118
