Karbek B
Diskapi Yildirim Beyazit Teaching and Research Hospital,
Ankara
Turkey
Research Article
The Effects of Cord Blood Serum on Survival of Rat Pancreatic Islets during In vitro Culturing
Author(s): Karbek B, Pinarli FA, Erden G, Cakal E and Delibasi TKarbek B, Pinarli FA, Erden G, Cakal E and Delibasi T
Background: Transplantation of islets of Langerhans can improve glycometabolic control in type 1 diabetic patients; however, recipients usually require islets from 1-3 donor pancreas. Current isolation techniques generally recover <50% of the islets from a given pancreas. This study aimed to determine the effect of cord blood serum (CBS) on isolated pancreatic islet cells in vitro.
Methods: Rat pancreatic islets were isolated via collagenase digestion, and then cultured with and without the presence of 50 μl, 100 μl, and 150 μl of CBS. Next, islet cell viability was determined using a fluorescein diacetate and propidium iodide mixture via fluorescence microscopy. Additionally, islet function was evaluated based on insulin secretion.
Results: Treatment of primary cultures of rat pancreatic islets with CB.. View More»
