Kavita R. Pandey
India
Research Article
Functional Characterization of Bacteriophage Resistant Mutants of Probiotic B. coagulans
Author(s): Kavita R. Pandey and Babu V. VakilKavita R. Pandey and Babu V. Vakil
Bacillus coagulans, a probiotic phage sensitive organism was subjected to random mutagenesis and several phage resistant mutants were isolated. Evaluation of mutants, in line with the WHO/FAO guidelines for probiotics, showed that Lactic acid production was not affected. Maximum sporulation efficiency was seen in mutant MIII (81% ± 2). Mutant MII showed least acid tolerance and tolerance to bile salts was lower in all the mutants compared to the parent (76% ± 0.5). All the cultures exhibited similar antibacterial activity (zones: 13-15 mm) against the pathogens used. For all the mutants bile salt hydrolase activity was positive and antibiotic susceptibility was similar to parental culture and all these mutants showed no hemolysis.
Auto-aggregation potential of the mutants was somewhat higher than the parental strain except for mutant PIII (29.5% &plusm.. View More»
