Kokkona Kouzi-Koliakou
Greece
Research Article
Application of a Novel High Yield Volume-Reduction Method for the
Cryopreservation of 950 UC Blood Units
Author(s): Nikos Tsagias, Kokkona Kouzi-Koliakou and George KoliakosNikos Tsagias, Kokkona Kouzi-Koliakou and George Koliakos
Umbilical cord blood represents an increasingly useful source of hematopoietic stem cells for Bone Marrow Transplantation (BMT), although currently, low cell numbers generally limit its use to pediatric patients. The aim of this study was to evaluate the routine use of a recently published volume reduction protocol for long term cord blood banking as was applied in a large number of cord blood samples. 950 cord blood samples with mean volume collected 78.81 ± 23.56 ml, Nucleated Cell (NC) 0.91 ± 0.44 × 109 NC, Monocytes (MN) 0.49 ± 0.23 × 109 MN and CD34+ HPCs (Haematopoietic Progenitors Stem Cells) 2.4 ± 2.08 × 106 CD34+ HPCs counts, have been collected respectively. We found a significant correlation between leukocyte counts and CD34+ HPCs with the volume collected (r=0.71, P<0.0001). We found a highl.. View More»
