Mahmoud Hashem
Egypt
Research Article
Molecular Study on Some Virulence and Fluoroquinolone Resistance Genes of Pseudomonas aeruginosa Isolated from Naturally Infected Cultured Sea Bream Fish (Sparus aurata) in Egypt
Author(s): Eman Khalifa, Mohamed Khallaf and Mahmoud HashemEman Khalifa, Mohamed Khallaf and Mahmoud Hashem
This study carried out to investigate the presence of some virulence and fluoroquinolone resistant genes by PCR and comparing with antibiotic diffusion disc for isolated P. aeruginosa. A total number of 100 alive cultured Sea bream, showing clinical signs were collected from a private fish farm at Damietta, Egypt then subjected to clinical, postmortem (PM), bacteriological examination, biochemical and serological identification of the isolated bacteria, P. aeruginosa were more prevalent and subjected to VITEK2 and PCR for detection of outer membrane lipoprotein gene (oprL) at "504 bp" and exotoxin A gene (toxA) at "270 bp" which indicated the virulent isolates of P. aeruginosa and DNA gyrAse (gyrA) at "287 bp" and topoisomerase IV (parC) at "267 bp" for determining fluoroquinolone resistance genes compared with antibiotic sensitivity test by disc diffusion using 3 of fluoroquinolone m.. View More»
