Mark A Crenshaw
Tanzania
Research Article
Lectin-Functionalized Magnetic Iron Oxide Nanoparticles for Reproductive Improvement
Author(s): Jean M Feugang, Shengfa F Liao, Mark A Crenshaw, Henry Clemente, Scott T Willard and Peter L RyanJean M Feugang, Shengfa F Liao, Mark A Crenshaw, Henry Clemente, Scott T Willard and Peter L Ryan
Background: Semen ejaculates contain heterogeneous sperm populations that can jeopardize male fertility. Recent development of nanotechnology in physiological systems may have applications in reproductive biology. Here, we used magnetic nanoparticles as a novel strategy for sperm purification to improve semen fertility.
Methods: Boar semen was obtained in artificial insemination doses from a local boar stud. Doses were mixed with or without magnetic nanoparticles designed to target and deplete moribund and poor performing spermatozoa under an electromagnetic field. Sperm motility characteristics were assessed prior to insemination of open gilts with control (n=3 gilts) and nanopurified (n=4 gilts) semen. Pregnancies were verified 30 days post-insemination. Litter sizes and post-natal development of piglets were respectively evaluated at part.. View More»
