Michelle M Sims
Tanzania
Research Article
Cytotoxicity of Hypo-osmolar Lavage Fluid on Ovarian Cancer Cells In vitro
Author(s): Elena M Paulus, Joseph T Santoso, Michelle M Sims, Jashmin K Patel and Lawrence M PfefferElena M Paulus, Joseph T Santoso, Michelle M Sims, Jashmin K Patel and Lawrence M Pfeffer
Objective: Ovarian cancer is difficult to diagnose early and many patients present with advanced disease. The presence of exfoliated ovarian cancer cells in the peritoneal cavity after debulking surgery is a poor prognostic indicator. Sterilization of the peritoneum during surgery may have clinical benefit in reducing tumor burden. Several studies have evaluated osmotic cytotoxicity in gastrointestinal and genitourinary cancers with varied results. We studied the cytotoxic effect of lavage fluids of differing osmolarities against multiple ovarian cancer cell lines (SKOV3, OV90, and OVCAR3) in vitro.
Methods: Cells were treated for either 10 minutes or 30 minutes with water, or 5 mOsm, 10 mOsm, 50 mOsm, 100 mOsm, 200 mOsm, 280 mOsm NaCl (dilutions in water), and PBS. After 24 hours, surviving cells were enumerated in a Coulter Counter... View More»
