Mithun Shaw
India
Research Article
Ecto-Cyclic AMP Independent Protein Kinase-A Potent Regulator of L-type Calcium Channel and Forward Motility of Goat (Capra indicus) Epididymal Spermatozoa
Author(s): Debjani Nath and Mithun ShawDebjani Nath and Mithun Shaw
Objective: Caprine (Capra indicus) spermatozoa possess a novel ecto-cAMP independent protein kinase (ecto- CIK) on their membrane surface. This enzyme showed remarkable alteration through the maturation process of spermatozoa in epididymis. We investigated the role of CIK in the regulation of forward motility of epididymal spermatozoa by controlling the intracellular level of [Ca2+].
Method: Cauda epididymal mature sperm cells were treated with CIK antibody and maximum inhibition of enzyme activity (85%) was observed at 120 minutes of exposure. To analyze the calcium uptake mechanisms cells were exposed to 45Ca2+ after treatment with CIK antibody and pretreated with different calcium channel regulators. The intracellular [Ca2+]i signal was determined fluorometrically by using fura 2-AM. The computerized spectrophotometric assay method was used .. View More»
