Journal of Multiple Sclerosis
ISSN - 2376-0389NLM - 101654564
Mitsunari Nakajima
School of Clinical Pharmacy,
4-2 Bunkyo-cho, Matsuyama 790-8578, Ehime
Japan
Research Article
Nobiletin Induces Oligodendrocyte Lineage Precursor Cells in a Cuprizone-Administered Demyelination Animal Model
Author(s): Mitsunari Nakajima, Risei Shimizu, Kohei Furuta, Mami Sugino, Takashi Watanabe, Rui Aoki, Satoshi Okuyama and Yoshiko FurukawaMitsunari Nakajima, Risei Shimizu, Kohei Furuta, Mami Sugino, Takashi Watanabe, Rui Aoki, Satoshi Okuyama and Yoshiko Furukawa
We investigated the effect of nobiletin on the oligodendroglial cell lineage in a cuprizone-induced demyelination animal model. Nobiletin, a polymethoxyflavone, was administered to the model animal for 3 consecutive weeks (50 mg/kg, intraperitoneally, 2 times/week). Immunohistochemical analysis of the corpus callosum and/or Western blotting analysis of brain extracts revealed that cuprizone administration reduced immunoreactivity for myelin-basic protein (MBP), a marker for mature oligodendrocytes, and increased immunoreactivity for platelet derived-growth factor receptor (PDGFR)-α, a marker for oligodendrocyte precursor cells (OPCs). Nobiletin treatment did not affect these changes in the cuprizone-fed mice. Nobiletin treatment, however, increased the immunoreactivity against proteolipid protein (PLP)/DM-20, a marker for mature oligodendrocytes, OPCs, and oligodendrocyte lineag.. View More»
