Pallop Pongsuthirak
Thailand
Research Article
Comparison of Medicult and Sage Media for In Vitro Maturation of Immature Oocytes Obtained during Cesarean Deliveries
Author(s): Pallop Pongsuthirak and Teraporn VutyavanichPallop Pongsuthirak and Teraporn Vutyavanich
Background: The success of in vitro maturation (IVM) of oocytes depends on many factors. Culture medium is one crucial part of the technique, but evidence-based selection of the medium is difficult because very few trials are available on this subject. In this study we compared two widely available commercial media, namely Medicult (Origo, Måløv, Denmark) and Sage medium (Cooper Surgical, Trumbull, CT, USA) for IVM of human oocytes.
Methods: One thousand and fifteen immature oocytes were collected by needle aspiration from ninety-three women, who underwent cesarean deliveries during a five-month period at a University Hospital in Thailand. Sibling oocytes were allocated to either Medicult (509 oocytes) or Sage IVM medium (506 oocytes) and assessed for maturation after 36 hours in culture. Mature oocytes were inseminated by intra.. View More»
