Pozzi CR
Brazil
Research Article
Effect of Gamma Radiation on Growth and Mycotoxin Production of Alternaria alternata
Author(s): Braghini R, Rocha LO, Pozzi CR, Frizzarin A, Reis TA and Corrêa BBraghini R, Rocha LO, Pozzi CR, Frizzarin A, Reis TA and Corrêa B
The ubiquitous genus Alternaria contains many species that are able to invade cereals, oleaginous plants and other crops. Alternaria alternata is considered one of the most important species and can produce several mycotoxins under favorable conditions of temperature and humidity, including the economically important toxins: alternariol (AOH) and alternariol monomethyl ether (AME). The aim of this study was to evaluate the radio-sensitivity of Alternaria alternata spores through different gamma radiation doses. A. alternata growth and the production of AOH and AME were then analyzed. After fungal irradiation with 2 kGy, 5 kGy and 7 kGy, the spores were suspended with sterile distilled water followed by inoculation on wheat grains. The count of the colony-forming units per gram (CFU/g) was performed using Dichloran Rose Bengal Chloramphenicol (DRBC) and Dichloran Chloramphenicol Agar M.. View More»
