Ralf Hofheinz
Germany
Research Article
LC/MS Based Monitoring of Endogenous Decay Markers for Quality
Assessment of Serum Specimens
Author(s): Jorg Oliver Thumfart, Nada Abidi, Sonani Mindt, Victor Costina, Ralf Hofheinz, Frank Klawonn, Michael Neumaier and Peter FindeisenJorg Oliver Thumfart, Nada Abidi, Sonani Mindt, Victor Costina, Ralf Hofheinz, Frank Klawonn, Michael Neumaier and Peter Findeisen
Introduction: Preanalytical variations have major impact on most biological assays. Specifically MS-based multiparametric proteomics analyses of blood specimens are seriously affected by limited protein stability due to high intrinsic proteolytic activity of serum and plasma. However, the direct analysis of sample quality (DASQ) for serum specimens is not readily available. Here we propose the mass spectrometry based monitoring of peptide patterns that are ex vivo changing in a time dependent manner to alleviate these constrains.
Materials and methods: Serum specimens from healthy controls (n=3) and patients with colorectal cancer were analyzed for a set of endogenous peptides (n=62). The respective proteolytic fragments were monitored with LC/MS at different preanalytical points in time ranging from 1 h to 48 h after blood withdrawal. An algorithm was constructed with a train.. View More»
DOI: 10.4172/jpb.1000356
