Sachin Patil
India
Research Article
Protective Effects of Diosgenin in Pentylenetetrazole Induced Kindling Model of Epilepsy in Mice
Author(s): Rufi Tambe, Pankaj Jain, Sachin Patil, Priya Ghumatkar and Sadhana SathayeRufi Tambe, Pankaj Jain, Sachin Patil, Priya Ghumatkar and Sadhana Sathaye
The present study was aimed at investigating the effects of Diosgenin, a plant derived steroidal sapogenin on the development and acquisition of pentylenetetrazole (PTZ) kindling along with altered biochemical parameters. Diosgenin (5 and 10 mg/kg, i.p.) was evaluated on the course of kindling development and its ability to suppress the PTZ induced oxidative damage in the brain tissue when given as a pretreatment prior to each PTZ injection. Various oxidative stress parameters such as superoxide dismutase (SOD), reduced glutathione (GSH), catalase (CAT) and lipid peroxidation (MDA) were assessed at the end of the study. Neuronal damage was studied by hematoxylin and eosin (H&E) staining technique. The neuroinflammatory marker glial fibrillary acidic protein (GFAP) was also evaluated at the end of the study using immunohistochemistry. Diosgenin significantly prevented the process o.. View More»
