Santosh Kumar
School of Pharmacy,
2464 Charlotte Street, Kansas City, MO 64108
Tanzania
Research Article
Analysis of Cytochrome P450 Conserved Sequence Motifs between Helices E and H: Prediction of Critical Motifs and Residues in Enzyme Functions
Author(s): Numan Oezguen and Santosh KumarNuman Oezguen and Santosh Kumar
Rational approaches have been extensively used to investigate the role of active site residues in cytochrome P450 (CYP) functions. However, recent studies using random mutagenesis suggest an important role for nonactive site residues in CYP functions. Meta-analysis of the random mutants showed that 75% of the functionally importantnon-active site residues are present in 20% of the entire protein between helices E and H (E-H) and conserved sequence motif (CSM) between 7 and 11. The CSM approach was developed recently to investigate the functional role of non-active site residues in CYP2B4. Furthermore, we identified and analyzed the CSM in multiple CYP families and subfamilies in the E-H region. Results from CSM analysis showed that CSM 7, 8, 10, and 11 are conserved in CYP1, CYP2, and CYP3 families, while CSM 9 is conserved only in CYP2 family. Analysis of different CYP2 subfamilies show.. View More»
