Sepp K
Hungary
Research Article
The Hormone Exocytosis in Prolactinoma and Normal Adenohypophysis Cell Cultures by the Effects of Hypocalcaemia
Author(s): Sepp K, László A, Radács M, Serester A, Valkusz Z, Gálfi M and Molnár ZSepp K, László A, Radács M, Serester A, Valkusz Z, Gálfi M and Molnár Z
The biological systems are opened, complex objects, which can regularly exchange feedbacks with their environment. The calcium ion is a universal messenger, which can regulate several cellular functions e.g. exocytosis machinery. The primary aim of this study was to investigate the response mechanisms of normal adenohypophysis and adenohypophyseal prolactinoma cell populations at different extracellular Ca2+ levels with an otherwise isoionic milieu of all other essential ions. We focused on prolactin (PRL) and adrenocorticotrophic hormone (ACTH) release. In our experimental study, female Wistar rats (n=10) were treated with estrone-acetate (150 μg/kg b.w/week) for 6 months to induce prolactinomas in the adenohypophysis. Primary, monolayer cell cultures were prepared by enzymatic and mechanical digestion. PRL and ACTH hormone presence was measured by radioimmunoassay or immuno- ch.. View More»
