Journal of Multiple Sclerosis
ISSN - 2376-0389NLM - 101654564
Simon Broadly
Australia
Research Article
A Pilot Investigation of Natural Killer Cell Function and Phenotypes in Stable and Active Multiple Sclerosis Patients
Author(s): Helene Cabanas, Natalie Eaton, Ellie Khalilidehkordi, Simon Broadly, Cassandra Balinas, Stanley Du Preez, Rebekah Maksoud, Donald Staines and Sonya Marshall-GradisnikHelene Cabanas, Natalie Eaton, Ellie Khalilidehkordi, Simon Broadly, Cassandra Balinas, Stanley Du Preez, Rebekah Maksoud, Donald Staines and Sonya Marshall-Gradisnik
Background: Previous studies have reported impaired cytotoxic activity in Natural Killer (NK) CD56Dim and CD56Bright cell phenotypes in peripheral blood associated with Multiple Sclerosis (MS). Recently it has been suggested that NK cell phenotype could be associated with new lesions on Magnetic resonance imaging (MRI) and may be used as an immunological indicator of disease activity. This project, for the first time, investigates NK cell cytotoxicity in MS patients with active and stable lesions.Methods: NK cell cytotoxic activity and NK CD56Dim and CD56Bright cell phenotypes were examined in MS patients using flow cytometry. Isolated NK cells were labelled with antibodies to determine CD56Dim and CD56Bright NK cells and cytotoxic function using target cells (K562). Seven patients (aged 38.0 ± 3.21) with stable Relapsing Remitting MS (RRMS.. View More»
