Suzanne M de la Monte
Pierre Galletti Research Building, Rhode Island Hospital,
55 Claverick Street, Room 419, Providence, RI 02903
Tanzania
Research Article
Comparative Analysis of Lipid Extracts and Imaging Mass Spectrometry for Evaluating Cerebral White Matter Biochemical Pathology in an Experimental Second-Hand Cigarette Smoke Exposure Model
Author(s): Alexander Krotow, Emine B Yalcin, Jared Kay and Suzanne M de la MonteAlexander Krotow, Emine B Yalcin, Jared Kay and Suzanne M de la Monte
Background: White matter injury and degeneration are common features of developmental and aging-associated diseases, yet their pathobiological bases are poorly understood. However, recent advances in Matrix-Assisted Laser Desorption Ionization (MALDI) instruments and chemistry have provided critical tools for myelin-lipid analytical research. Design: This study characterizes Cigarette Smoke (CS) exposure effects on frontal lobe lipid ion profiles in adult male A/J mice that had been exposed to air for 8 weeks (A8), CS for 4 (CS4) or 8 weeks (CS8), or CS8 followed by 2 weeks recovery (CS8+R). MALDI data acquired by analysis of lipid extracts plated onto a ground steel target (high through-put) were compared with Imaging Mass Spectrometry (IMS). Results: MALDI-time-of-flight (TOF) detected 120 lipid ions with m/z?s of 600 to 1300 (phospholipids and sulfatides) in samples plated onto the st.. View More»
