Thorvald Löfkvist
Sweden
Research Article
Evaluation of Skin Reactivity during (Immuno-) Therapy. Validation of Methods for Estimation of Changes in Skin Reactivity and Correlation to Shock Organ Sensitivity
Author(s): Sten Dreborg, Antonio Basomba, Thorvald Löfkvist, Margareta Holgersson and Christian MöllerSten Dreborg, Antonio Basomba, Thorvald Löfkvist, Margareta Holgersson and Christian Möller
Background: Parallel line bioassay (PLBA) has been acknowledged to be the gold standard for estimation of changes in reactivity, e.g., in RAST and ELISA inhibition tests.
Objective: To study correlations between two simple methods for evaluation of changes in skin prick test (δSPT), using the slope of the allergen dose response (drra) in relation to PLBA.
Methods: Skin prick test data from two published immunotherapy trials were used. In a D. farinae trial we used duplicate tests with three fixed ten-fold concentrations and in a P. judaica trial three tenfold individually chosen allergen concentrations causing wheals of similar size to that of histamine dihydrochloride 10 mg/mL, tenfold lower and tenfold higher concentration. Evaluation of the δSPT by PLBA, and two simple me.. View More»
