Toshihiko Yamashita
Japan
Research Article
A Novel Rat Model of Ankle Osteoarthritis Induced by the Application of Monoiodoacetate
Author(s): Syunsuke Jimbo, Yoshinori Terashima, Tsuneo Takebayashi, Atsushi Teramoto, Izaya Ogon, Kota Watanabe, Tatsuya Sato, Nobutoshi Ichise, Noritsugu Tohse and Toshihiko YamashitaSyunsuke Jimbo, Yoshinori Terashima, Tsuneo Takebayashi, Atsushi Teramoto, Izaya Ogon, Kota Watanabe, Tatsuya Sato, Nobutoshi Ichise, Noritsugu Tohse and Toshihiko Yamashita
Although ankle osteoarthritis is a common human joint disease, the etiological mechanisms underlying the associated pain remain unknown. We developed a new rat model of ankle osteoarthritis (OA) induced by monoiodoacetate (MIA) and evaluated the behavioral and histological characteristics of these animals. 60 male Sprague-Dawley rats were divided into three experimental groups of 20 rats. OA was induced by injecting the metabolic inhibitor MIA (1 mg/30 μl) into the tibiotarsal joint of the right hind paw on two consecutive days (MIA2 group) or single day (MIA1 group). Saline was injected into sham rats using the same protocol (sham group). We examined ankle swelling, range of motion, and stride length. We also assessed pain related behavior by observing the mechanical and thermal withdrawal responses. Rats were sacrificed 28 days after MIA/saline injection, and the right ankle jo.. View More»
