Wolfgang Schlickewei
Germany
Research Article
Mitochondrial DNA Deletions are Associated with Upregulated Articular Nitrotyrosine in Human Femoral Head Cartilage
Author(s): Jörn Zwingmann, Bernhard Setzer, Markus Große Perdekamp, Wolfgang Schlickewei, Norbert P Südkamp, Chingching Foocharoen and Ulrich A WalkerJörn Zwingmann, Bernhard Setzer, Markus Große Perdekamp, Wolfgang Schlickewei, Norbert P Südkamp, Chingching Foocharoen and Ulrich A Walker
Background: The pathogenesis of osteoarthritis (OA) has been linked with the elaboration of increased amounts of nitrotyrosine in cartilage as a stable marker of tyrosine nitration by nitric oxide. Reactive oxygen species (ROS) are known to damage mitochondrial DNA (mtDNA); this process is associated with aging of non-cartilaginous tissues. This study aimed to link intraarticular nitrotyrosine content, with mtDNA lesions and OA severity in order to gain insights into the contribution of nitrotyrosine and mitochondria to cartilage aging and the development of OA.
Methods: Femoral heads were prospectively obtained from individuals undergoing hip arthroplasty or postmortem autopsy (22 male, 26 female, age range 16-93 years). OA severity was graded radiographically, and histologically by means of the Mankin score. Articular chondrocytes were iso.. View More»
