Graduate School of Science and Technology, Niigata University, Niigata, Japan
Research Article
Inhibition of 20?-Hydroxysteroid Dehydrogenase Activity in Mouse Oocyte by
Human 20?-HSD (AKR1C1) Inhibitor
Author(s): Shin-ya Kawakami*, Chihiro Nansai and Sueo Niimura
Inhibitors of the enzyme 20α-HSD (AKR1C18) are required to investigate AKR1C18 activity that occurs with meiotic resumption and progression in mouse oocytes. However, currently, no effective inhibitors of AKR1C18 are available. Therefore, we treated mouse oocytes with 3-bromo-5-phenylsalicylic acid (5-PBSA), an inhibitor of human 20α-HSD (AKR1C1), to ascertain whether it suppressed AKR1C18 activity. We also examined the effect of this inhibitor on oocyte maturation and viability.
In the germinal vesicle stage of mouse oocytes treated with 300 and 400 μM 5-PBSA, the number of oocytes showing AKR1C18 activity in the cytoplasm was 0 (0/34 and 0/35); the activity was significantly suppressed as compared with AKR1C18 activity in the control (25/29, 86.2%). In oocytes treated with >200 μM 5-PBSA, the number of degenerated oocytes was significantly increased co.. View More»
