Journal of Multiple Sclerosis
ISSN - 2376-0389NLM - 101654564
Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran
Research
Role of B Cells and Myelin Basic Protein-Induced Antibodies in the Pathogenesis of Experimental Autoimmune Encephalomyelitis in C57BL/6 J Mice
Author(s): Sherman Maryam Sahlolbei, Maryam Hajizadeh, Marzieh Naseri, Leili Saeednejad Zanjani, Hossein Ghanbarian, Zahra Madjd, Seyed Mahmoud Hashemi and Jafar Kiani*
Background: Over the years, the treatment of multiple sclerosis (MS) has improved due to the dramatic advancement in immunology and neuroscience. The recent therapeutic approaches substantially expanded our view of the role of B cells in the progression of MS. The present study aimed to investigate the role of B cells and Myelin Basic Protein (MBP)-induced antibodies in developing Experimental Autoimmune Encephalomyelitis (EAE, an animal model of multiple sclerosis).
Method: We used MBP for induced experimental autoimmune encephalomyelitis in C57BL/6 female mice and analyzed the clinical score. The Enzyme-Linked Immunosorbent Assay (ELISA) and western blot assessed the expression of MBP-induced antibodies. The infiltration rate of the inflammatory cells and demyelination in the spinal cord were also evaluated by Hematoxylin/Eosin, Luxol fast.. View More»
DOI:
10.35248/2376-0389.23.10.4.491
