Department of Molecular Biology and Genetics, University of Gadjah Mada, Yogyakarta, Indonesia
Review Article
Agrobacterium-Mediated Transformation Improvement in Tomato
Author(s): Jawad* and Sadiq Ali
In this article, we describe a highly efficient and reproducible method of Agrobacterium-mediated transformation protocol for several gene transformations (cry1Ab, rolB, and PhMV) in tomatoes. Tomato fruit is rich in minerals, essential amino acids, sugar, vitamin B, iron, phosphorus, and dietary fiber. Tomato Crop production has some major problem which includes early ripening, reduction in fruit shelf life and devastating diseases that caused by viruses, bacteria, fungi, and nematodes, etc. The study aims to use the Agrobacterium-mediated transformation method to improve the tomato shelf life and make tomato plants resistant to such types of diseases caused by bacteria, viruses, and fungi, etc. Tomato was transformed via Agrobacterium tumefaciens strain LBA4404 harbouring Cry1Ab synthetic gene for toxin proteins. And sa.. View More»
DOI:
10.35248/2322-3308.21.10.017
