Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, Novosibirsk, Russia
Research Article
Therapeutic Potential of Fibroblast Combined with Platelet-Rich Plasma on Burn Skin Wound Healing
Author(s): Alexander Petrovich Lykov*, Olga Vladimirovna Poveshchenko, Natalia Anatollievna Bondarenko and Maria Alexandrovna Surovtseva
Fibroblasts and platelet-rich plasma (PRP) used in regenerative medicine. This study evaluated whether fibroblast alone or in combination with PRP promote burn skin wound healing in vivo. Twenty CBA male mice were divided to 4 treatment groups: control (0.05 mL of phosphate-buffered saline, intradermal), PRP (0.05 mL of PRP, intradermal), fibroblasts/PRP (4x104 fibroblast in 0.05 mL PRP, intradermal), and fibroblasts (4x104 fibroblast in 0.05 mL PBS, intradermal), immediately after burning with a metal rod (100 °C for 40 s). The healing process was evaluated by gross examination throughout the experimental period on day 4, 11, 14 and 21, and histologic examination on day 21. Cytokines, growth factors and NO were determined in PRP by ELISA. The results indicated that the combination fibroblasts with PRP significantly promote wound healing (p ≤ 0.05). Histol.. View More»
