Hanshuang Zhang, Jianguang Liu, Yongqiang Wang, B.S, Institute of Cotton Research, Hebei,China Guiyuan Zhao, Junli Zhao, Haiying Du and Xiaoliang He
Institute of Cotton Research, China
Posters & Accepted Abstracts: J Plant Biochem Physiol
Ethylene-responsive factors (ERFs) are commonly considered to play important role in pathogen defense responses. However, only few of ERF members have been characterized in Sea island cotton (Gossypium barbadense). Here we reported a novel AP2/ERF transcription factors gene, named GbERFb which was cloned and identified from Gossypium barbadense by RACE. The expression of GbERFb was significantly up-regulated by ET, MeJA, SA, wounding, H2O2 and Verticillium dahliae. Bioinformatics analysis showed that GbERFb protein contain a conserved ERF DNA binding domain and a nuclear localization signal sequence. Further exprements demonstrated that GbERFb could be bind to GCC box cis-acting element and interact with the GbMAPKb (MAP kinase) directly in yeast. Over-expression of GbERFb in tobacco could increase the disease resistance to V.dahliae. These results suggest that the GbERFb, encode an AP2/ERF transcription factor, could be utilized for enhancing V.dahliae tolerance. Recent Publications 1. Meng, X., Li, F., Liu, C., Zhang, C., Wu, Z. and Chen, Y. (2010) Isolation and Characterization of an ERF Transcription Factor Gene from Cotton (Gossypium barbadense L.). Plant Mol Biol Rep, 28, 176-183. doi:10.1007/s11105-009-0136-x 2. Guo, W., Jin, L., Miao, Y., He, X., Hu, Q., Guo, K., Zhu, L. and Zhang, X. (2016) An ethylene response-related factor, GbERF1-like, from Gossypium barbadense improves resistance to Verticillium dahliae via activating lignin synthesis. Plant Mol Biol, 91, 305-318. doi:10.1007/s11103-016-0467-6 3. Li, C., He, X., Luo, X., Xu, L., Liu, L., Min, L., Jin, L., Zhu, L. and Zhang, X. (2014) Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton by Verticillium dahliae by Activating JASMONATE ZIM-DOMAIN1 Expression. Plant Physiol, 166, 2179-2194. doi:10.1104/pp.114.246694 4. Cai, Y., Xiaohong, H., Mo, J., Sun, Q., Yang, J. and Liu, J. (2012) Molecular research and genetic engineering of resistance to Verticillium wilt in cotton: A review. African Journal of Biotechnology, 8, 7363-7372. 5. Zhang, Z., Zhao, J., Ding, L., Zou, L., Li, Y., Chen, G. and Zhang, T. (2016) Constitutive expression of a novel antimicrobial protein, Hcm1, confers resistance to both Verticillium and Fusarium wilts in cotton. Sci Rep-Uk, 6, 20773. doi:10.1038/srep20773.
Hanshuang Zhang, professor, female, born in Hebei province. China. She has engaged in cotton breeding for over 20 years, and won the Technological Progress First Prize Award of Hebei province. Her team has successively presided and finished national or provincial cotton research projects more than 30 items, and breeding nearly 10 high-yielding, disease-resistant and high-quality cotton varieties, such as Jimian169, JiH 170, Ji178 etc.
E-mail: hanshuangzhang@126.com
