Javier Del Pino, Paula Moyano, Maria Teresa Frejo, Maria Jesus Diaz, Gloria Gomez, Maria Jose Anadon, Margarita Lobo, Jimena Garcia, Miguel Andres Capo and Jose Manuel Garcia
Complutense University, Spain
Alfonso X University, Spain
Posters & Accepted Abstracts: J Clinic Toxicol
Chlorpyrifos (CPF) is an organophosphate insecticide reported to induce both after acute and repeated exposure learning and memory dysfunctions, although the mechanism is not completely known. CPF produces basal forebrain cholinergic neuronal loss, involved on learning and memory regulation, which could be the cause of such cognitive disorders. Otherwise, neuronal dendritic spines were reported to be also involved on learning and memory process regulation and their alteration could also contribute to this effect. In this regard, CPF has been reported to induce an alteration in the dendritic spines density in the prefrontal cortex and hippocampus after acute and repeated exposure to subclinical doses respectively, thus their alteration in basal forebrain cholinergic neurons could also mediate cognitive disorders. Accordingly, we hypothesized that CPF induces basal forebrain cholinergic dendritic spine alteration at low concentrations and at higher concentrations produces cell death. We evaluated in septal SN56 basal forebrain cholinergic neurons, the CPF effect after 24 h and 14 days exposure on dendritic spines. This study shows that CPF induces after acute and long-term exposure an alteration of dendritic spines at lower concentrations than which induces cell death. Evaluation of genes related to dendritic spine plasticity revealed that some of them are altered at lower concentrations than which produces the effects observed and below the No Observed Adverse Effect (NOAEL). The present finding suggests that the use of gene expression profile could be a more sensitive and accurate way to determine the NOAEL.
Javier Del Pino received his PharmD degree at the University Complutense University of Madrid in 2004. He has two Master’s in Sciences 2009 and 2010. He specialized in neurotoxicology and neurodevelopmental toxicology and received his PhD in Toxicology in 2009. In 2010, he worked in Institute of Health Carlos III in the National Center of Environmental Health. From 2010 to 2012, he was Associated Researcher at University of Massachusetts (UMASS) working in Sandra Petersen´s Lab in a National Institute of Health (NIH) project on developmental effects of TCDD endocrine disruptor on sexual differentiation. In 2016, he got a position as Associated Professor of Toxicology at the Complutense University of Madrid.
Email: jdelpino@pdi.ucm.es
