Daniela Oliveira
AcceptedAbstracts: J Clinic Toxicol
T he choice of Baccharis for this project was based observing phytochemical study with their extracts, which showed various biological effects such as antimicrobial, cytotoxic and anti-inflammatory drugs mainly. Thus, due to the great importance for the medicine, commercial and ecological, several species of Baccharis have focus of multidisciplinary groups involving chemical, biological and pharmacological researchers. Our study evaluated the effect of the methanol extract of Baccharis microdonta partition and its phases on the enzymatic activity and pharmacological activity sPLA2 isolated from the Bothrops jararacussu venom. sPLA2 was isolated by combination of two chromatographic procedures started with ion exchange chromatography (TSK Gel SP 5PW) and final purification step was done in C5 reverse phase HPLC. The plant material of B. microdonta (aereal parts) was dried and it was then subjected to extraction with high polarity solvents (ethanol). The resulting extract was then, subjected to partition steps of liquid/liquid using for both different solvents, such as hexane, dichloromethane, ethyl acetate and n-butanol. The enzymatic activity was measured using a NOB as synthetic substrate and enzymatic activity of the sPLA2 was determined by measurement of product formation monitored at A425nm. Pharmacological assays were performed with preincubation of PLA2 with the extract and partition obtained from methanolic extract of B. microdonta . The n-butanol phase was the only one that showed a significant reduction in the enzymatic activity of PLA2. The paw edema test in mice methanolic extract and phase in dichloromethane significantly reduced edema. Myotoxic PLA2 activity was reduced with ethyl acetate phase. With these results we found that the extract methanolic and dichloromethane phase of B. microdonta presents promising in fighting inflammatory activity. This result suggests that such activity is not related to the reduction of PLA2, but with decreased synthesis of COX-I, whose results were verified using real-time PCR
Daniela Oliveira was graduated in Dentistry from the University San Francisco, master, doctorate and postdoctorate in Biochemistry from the State University of Campinas-São Paulo-Brazil. She is a professor and researcher at the Mackenzie Presbyterian University, his area of research is the search for anti-inflammatory molecule of plant origin whose target is the snake sPLA
