Birru Bhaskar, Santhosh Kacham, Naveen Kumar Mekala and Parcha Sreenivasa Rao
National Institute of Technology, India
Posters & Accepted Abstracts: J Cell Sci Ther
Bone tissue engineering aims to develop engineered bone tissue to substitute the conventional bone graft and to produce an unlimited supply of engineered constructs to meet the present global demand. To achieve this, culturing the cells on biocompatible three-dimensional (3D) scaffold is essential. The major limitations for functional tissue generation in 3D culture such as limited knowledge on the physicochemical parameters, regulatory mechanism of the tissue development and the cost of the polymers are yet to be addressed. Introduction of bioreactor system in tissue engineering addressed many challenges in this field. Bioreactor system provides the balanced microenvironment with optimal physical and chemical parameters resulting in improved proliferation, differentiation and production of functional tissue. Various types of bioreactors were developed and evaluated for the purpose of developing engineered tissue grafts. Significant differences noticed in the tissues developed and the problems aroused during the tissue development, lead to the invention of perfusion flow bioreactor system. Mechanical loading on the cells in perfusion reactor system at ex vivo level showed higher proliferation and differentiation of Mesenchymal Stem Cells (MSCs) that is quantified by ALP assay, cell morphological studies, mRNA expression of bone morphogenic protein (BMP) and DNA quantification. The perfusion system is capable of producing multiple tissue engineered constructs with the uniform cell distribution. Compared to other bioreactors it is easy to handle and is being effectively operated in the development of functional tissue for clinical use to meet the global demand.
Email: bhaskar2314@nitw.ac.in
